Circulating mir-200c and mir-33a may be used as biomarkers for predicting high fructose corn syrup-induced fatty liver and vitamin D supplementation-related liver changes

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dc.authoridSirin, Cansin/0000-0002-4530-701X
dc.authorwosidSirin, Cansin/ABI-3816-2020
dc.contributor.authorTanoğlu, Alpaslan
dc.contributor.authorÇağıltay, Eylem
dc.contributor.authorTanoğlu, Esra Güzel
dc.contributor.authorGökhan, Aylin
dc.contributor.authorŞirin, Cansın
dc.contributor.authorÇavusoğlu, Türker
dc.contributor.authorYeşilbaş, Soner
dc.date.accessioned2023-03-22T19:47:18Z
dc.date.available2023-03-22T19:47:18Z
dc.date.issued2022
dc.departmentBelirleneceken_US
dc.description.abstractBackground/aim: Nonalcoholic fatty liver is one of the most common forms of liver disease and role of microRNAs (miRNAs) on this illness is currently unclear. It was aimed to evaluate the predictive role of circulating miR-33a and mir-200c on high fructose corn syrup (HFCS)-induced fatty liver and vitamin D-3 supplementation-related hepatic changes. Materials and methods: Twenty-four rats were randomized into three groups: sham (n = 8), experimental fatty liver group (n = 8), and fatty liver + vitamin D-3 supplementation group (n = 8). In the treatment group, 10 mu g/kg/week of vitamin D-3 was given by orogastric tube weekly for 4 weeks in addition to a high fructose diet. Serum AST, ALT, TNF-alpha, and MDA levels were tested. Liver tissue samples were examined using hematoxylin/eosin, periodic acid-Schif (PAS) and Masson's Trichrome staining. Circulating miR-33a and mir-200c were quantified by qRT-PCR method. Moreover, in silico analyses were accomplished. Results: In the vitamin D-3 group, results of biochemical parameters were significantly different than those of the fatty liver group (p < 0.001). Moreover, significant differences in serum levels of circulating miR-33a and mir-200c were identified among all groups (p < 0.05). Finally, more favorable histopathological changes were noticed in the vitamin D-3 supplementation group. The expressions of Ki-67 were also considerably reduced in the vitamin D-3 group. According to KEGG pathway analysis, mir-33a and mir-200c were found to play a common role in the Hippo signaling pathway, lysine degradation, and protein processing. Conclusion: Our current experimental fatty liver study showed that, in a specified dose, vitamin D-3 supplementation could alleviate adverse undesirable hepatic effects of HFCS via miR-33a and mir-200c.en_US
dc.description.sponsorshipScientific Research Project Fund of University of Health Sciences [2018/024]en_US
dc.description.sponsorshipThis work is supported by the Scientific Research Project Fund of University of Health Sciences under the project number 2018/024.en_US
dc.identifier.doi10.55730/1300-0144.5483
dc.identifier.endpage1457en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue5en_US
dc.identifier.pmid36422507en_US
dc.identifier.scopus2-s2.0-85140291179en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1448en_US
dc.identifier.trdizinid1145063en_US
dc.identifier.urihttps://doi.org/10.55730/1300-0144.5483
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1145063
dc.identifier.urihttps://hdl.handle.net/20.500.14034/605
dc.identifier.volume52en_US
dc.identifier.wosWOS:000890907200003en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherScientific And Technological Research Council Turkeyen_US
dc.relation.journalTurkish Journal Of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFatty liveren_US
dc.subjectvitamin D-3en_US
dc.subjectmicroRNAen_US
dc.subjectbiomarkeren_US
dc.subjectraten_US
dc.subjectInsulin-Resistanceen_US
dc.subjectExpressionen_US
dc.subjectCholesterolen_US
dc.subjectRatsen_US
dc.subjectInflammationen_US
dc.subjectContributesen_US
dc.subjectDeficiencyen_US
dc.subjectDiseaseen_US
dc.subjectAlphaen_US
dc.subjectRisken_US
dc.titleCirculating mir-200c and mir-33a may be used as biomarkers for predicting high fructose corn syrup-induced fatty liver and vitamin D supplementation-related liver changesen_US
dc.typeArticleen_US

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