Effect of Colchicine on Secondary Acute Lung Injury in an Experimental Sepsis Model in Rats
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Dosyalar
Tarih
2023
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Dergi ISSN
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Yayıncı
Galenos Publ House
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Introduction: An irregular systemic inflammatory response in sepsis can induce acute lung injury (ALI), which is characterized by the exaggerated inflammation of the lung tissue. Colchicine inhibits several inflammatory pathways which may prevent sepsis-induced secondary lung injury. We aimed to examine the histopathological effects of colchicine on lung tissue in a lipopolysaccharide (LPS)-induced sepsis model in rats.Materials and Methods: Twenty-eight rats were randomly divided into the following four groups: sham (n=6), colchicine only (n=6), sepsis only (n=8) and sepsis+colchicine (n=8). In the sham group, 0.9% NaCl was administered intraperitoneally (IP) and intragastrically (IG). In the Colchicine only group, 0.9% NaCl was administered IP and colchicine was administered IG. Sepsis was induced in the sepsis only and sepsis+colchicine groups by administering of 1 mg/kg of LPS IP at the 0-time point. In the sepsis+colchicine group, colchicine was additionally given IG at the 90th minute. An observational sepsis scoring system was used to evaluate the signs of sepsis. Subsequently, the rats were sacrificed at the 24th hour. The lung tissues were examined according to the American Thorax Association's assessment report on ALI in animals.Results: The histopathological lung injury score in the sepsis+colchicine group was significantly higher than in the sham group (0.23 +/- 0.18 vs. 0 +/- 0; p<0.05) and was significantly lower than in the sepsis only group (0.23 +/- 0.18 vs. 0.57 +/- 0.14; p<0.001). Evaluation of the lung damage score revealed that colchicine suppressed the increase in alveolar and interstitial neutrophil counts and limited the increase in hyaline membrane counts and alveolar wall thickness in the sepsis+colchicine group when compared with the sepsis only group.Conclusion: In our experimental sepsis rat model, administration of colchicine for sepsis limited the secondary lung damage.
Açıklama
Anahtar Kelimeler
Sepsis; Acute Lung Injury; Colchicine; Rat Model
