Investigation of changes in liver microanatomy in the steatosis model created by permanent canula in rats

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dc.contributor.authorNalbant, Asrın
dc.contributor.authorSakul, B. Ufuk
dc.contributor.authorYucel, Ferruh
dc.date.accessioned2023-03-22T19:47:18Z
dc.date.available2023-03-22T19:47:18Z
dc.date.issued2022
dc.departmentBelirleneceken_US
dc.description.abstractObjective: The knowledge of nonalcoholic fatty liver disease (NAFLD) and Nonalcoholic Steatohepatitis (NASH) is limited to the findings from available suitable models for this disease. A number of rodent models have been described in which relevant liver pathology develops in an appropriate metabolic context. In this experimental study, it was aimed to create a new liver fat model by giving fat from the portal vein of rats and to visualize the changes in the liver with advanced microscopic techniques.Methods: 28 female rats were used in the study. Permanent intraabdominal cannulas were inserted into the portal vein of the rats. Rats were randomly divided four group. Intralipid 20% substance was injected through cannula to the experimental groups during the test period. Control group received saline at the same rate. At the end of the experiment, the animals were visualized with a laser speckle microscope and livers were divided into sections according to the stereological method. The sections were painted with Hematoxylin-Eosin, Oil red o, Masson trichoma, Bodipy, Nile red. Sections were evaluated under a microscope.Results: Ballooning, inflammation and fibrosis were observed in the 2 week intralipid group. In the 1 week intralipid group, the rate of parenchyma decreased while the sinusoid rate increased, and sinusoid rate increased significantly in the 2 week intralipid (p<0.05). Conclusion: According to the findings, steatohepatitis was detected in the 2 week intralipid, whereas only steatosis was observed in the 1 week intralipid. Thus, it was concluded that the newly formed rat model causes steatosis.en_US
dc.identifier.doi10.33808/clinexphealthsci.948391
dc.identifier.endpage664en_US
dc.identifier.issn2459-1459
dc.identifier.issue3en_US
dc.identifier.startpage659en_US
dc.identifier.trdizinid1127937en_US
dc.identifier.urihttps://doi.org/10.33808/clinexphealthsci.948391
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1127937
dc.identifier.urihttps://hdl.handle.net/20.500.14034/597
dc.identifier.volume12en_US
dc.identifier.wosWOS:000863981200017en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherMarmara Univ, Inst Health Sciencesen_US
dc.relation.journalClinical And Experimental Health Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectNAFLDen_US
dc.subjectSteatosisen_US
dc.subjectPortal veinen_US
dc.subjectConfocal microscopeen_US
dc.subjectLaser speckleen_US
dc.subjectAnimal-Modelsen_US
dc.subjectNonalcoholic Steatohepatitisen_US
dc.subjectPortal-Veinen_US
dc.subjectDiseaseen_US
dc.titleInvestigation of changes in liver microanatomy in the steatosis model created by permanent canula in ratsen_US
dc.typeArticleen_US

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