Longitudinal Investigation of Brain and Spinal Cord Pericytes After Inducible PDGFRβ+ Cell Ablation in Adult Mice

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dc.contributor.authorAtak, Dila
dc.contributor.authorYildiz, Erdost
dc.contributor.authorOzkan, Esra
dc.contributor.authorYousefi, Mohammadreza
dc.contributor.authorOzkan, Ayse
dc.contributor.authorYilmaz, Aysu Bilge
dc.contributor.authorKizilirmak, Ali Burak
dc.date.accessioned2025-03-20T09:50:47Z
dc.date.available2025-03-20T09:50:47Z
dc.date.issued2025
dc.departmentİzmir Bakırçay Üniversitesi
dc.description.abstractCentral nervous system (CNS) pericytes play crucial roles in vascular development and blood-brain barrier maturation during prenatal development, as well as in regulating cerebral blood flow in adults. They have also been implicated in the pathogenesis of numerous neurological disorders. However, the behavior of pericytes in the adult brain after injury remains poorly understood, partly due to limitations in existing pericyte ablation models. To investigate pericyte responses following acute ablation and characterize a novel rodent model for pericyte research, we developed a tamoxifen-inducible PDGFR beta(+) cell ablation model by crossing PDGFR beta-P2A-CreER(T2) and Rosa26-DTA176 transgenic mouse lines. Using this model, we studied the effects of different tamoxifen doses and conducted histological examinations 15 and 60 days post-injection to assess the impacts of PDGFR beta(+) cell ablation in both acute and chronic phases, respectively. Our results demonstrate that a low dose of tamoxifen effectively ablates PDGFR beta(+) cells of the CNS in mice without reducing survival or causing significant systemic side effects, such as weight loss. Additionally, we found that the extent of PDGFR beta(+) cell depletion varies between the cortex and the spinal cord, as well as between the gray and white matter regions of the spinal cord. Importantly, we observed that both pericyte coverage and numbers increased in the weeks following acute ablation, indicating the regenerative capacity of CNS pericytes in vivo. This study offers a valuable tool for future studies on the role of pericytes in neurological disorders by overcoming the limitations of constitutive pericyte ablation models and providing its longitudinal characterization in the CNS.image
dc.description.sponsorshipTUBIdot;TAK [116S252]; TUBIdot;TAK 2236 Co-Funded Brain Circulation Scheme 2 [119C018]
dc.description.sponsorshipThis study was funded by TUB & Idot;TAK (project no: 116S252). Dr. Atay Vural was supported by TUB & Idot;TAK 2236 Co-Funded Brain Circulation Scheme 2 (Project No: 119C018).
dc.identifier.doi10.1111/jnc.70035
dc.identifier.issn0022-3042
dc.identifier.issn1471-4159
dc.identifier.issue3
dc.identifier.pmid40066845
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1111/jnc.70035
dc.identifier.urihttps://hdl.handle.net/20.500.14034/2316
dc.identifier.volume169
dc.identifier.wosWOS:001441240600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Neurochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250319
dc.subjectbrain
dc.subjectcortex
dc.subjectinducible PDGFR beta(+) cell ablation
dc.subjectregeneration
dc.subjectspinal cord
dc.subjecttamoxifen
dc.subjecttransgenics
dc.titleLongitudinal Investigation of Brain and Spinal Cord Pericytes After Inducible PDGFRβ+ Cell Ablation in Adult Mice
dc.typeArticle

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