First dose optimization study on freezing Anatolian buffalo semen

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dc.authoridKaya, Ufuk/0000-0002-4805-0993
dc.authoridBastan, ilktan/0000-0001-8155-1960
dc.contributor.authorBastan, Ilktan
dc.contributor.authorSahin, Derya
dc.contributor.authorKorkmaz, Firat
dc.contributor.authorSimsek, Seher
dc.contributor.authorKaya, Ufuk
dc.contributor.authorSatilmis, Muharrem
dc.date.accessioned2025-03-20T09:50:30Z
dc.date.available2025-03-20T09:50:30Z
dc.date.issued2024
dc.departmentİzmir Bakırçay Üniversitesi
dc.description.abstractThe main objective of sperm production centers is to produce as many straws as possible from the obtained ejaculates using the optimal dilution rate. To this end, this study is the first to evaluate the effect of different semen extender rates on Anatolian buffalo semen quality. Ejaculates were collected by artificial vagina from three Anatolian buffalo bulls. These ejaculates were divided into three aliquots and filled into 0.25 ml straws with soy-based extenders at concentrations of 35, 25, and 15 million sperm/straw (n=105). The straw samples of different sperm concentrations were frozen. The quality of sperm was evaluated after thawing (37 degrees C, 30 sec) and following the thermoresistance test (37 degrees C, 3 h). The post-thaw total motility and progressive motility values were similar between the groups. However, following the thermoresistance test, there was a significant decrease in total motility in the 35 million sperm/straw group, and the progressive motility was significantly higher in the 25 million sperm/straw group. There was no statistically significant difference between the groups in terms of sperm kinetic parameters, except for VSL after thawing, as well as VAP and LIN values following the thermoresistance test. The overall mean PMAI and STR values were the highest in the 25 million sperm/straw group. In conclusion, it is recommended to dilute the Anatolian buffalo semen at a concentration of 25 million/0.25 ml when freezing it with a soy-based semen extender. In addition, it is considered that soy-based extenders compensate for cryo-damage to sperm motility for a short time, and the thermoresistance test should be applied for objective evaluation in dose optimization studies.
dc.description.sponsorshipDirectorate of the International Center for Livestock Research and Training Institute, Ankara, Turkiye; Department of Biotechnology and Artificial Insemination, the International Center for Livestock Research and Training Institute
dc.description.sponsorshipThis study was supported by the Directorate of the International Center for Livestock Research and Training Institute, Ankara, Turkiye. The authors thank the Department of Biotechnology and Artificial Insemination, the International Center for Livestock Research and Training Institute for their support and assistance.
dc.identifier.doi10.33988/auvfd.1205794
dc.identifier.endpage356
dc.identifier.issn1300-0861
dc.identifier.issn1308-2817
dc.identifier.issue3
dc.identifier.scopus2-s2.0-85201830401
dc.identifier.scopusqualityQ3
dc.identifier.startpage349
dc.identifier.trdizinid1249219
dc.identifier.urihttps://doi.org/10.33988/auvfd.1205794
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1249219
dc.identifier.urihttps://hdl.handle.net/20.500.14034/2228
dc.identifier.volume71
dc.identifier.wosWOS:001282046100002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherAnkara Univ
dc.relation.ispartofAnkara Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250319
dc.subjectBubalis bubalis
dc.subjectComputer-assisted sperm analysis
dc.subjectFlow cytometry
dc.subjectFrozen semen
dc.subjectSemen extender
dc.titleFirst dose optimization study on freezing Anatolian buffalo semen
dc.typeArticle

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