Effects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservation

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dc.authoridTomruk, Canberk/0000-0002-3810-3705
dc.authoridGökhan, Aylin/0000-0002-6254-157X
dc.authoridSirin, Cansin/0000-0002-4530-701X
dc.authorwosidTomruk, Canberk/AAN-4976-2020
dc.authorwosidGökhan, Aylin/AAE-2494-2019
dc.authorwosidSirin, Cansin/ABI-3816-2020
dc.contributor.authorGokhan, Aylin
dc.contributor.authorCavusoglu, Tuerker
dc.contributor.authorKilic, Kubilay Dogan
dc.contributor.authorSirin, Cansin
dc.contributor.authorTomruk, Canberk
dc.contributor.authorYigitturk, Guerkan
dc.contributor.authorErbas, Oytun
dc.date.accessioned2024-03-09T18:48:51Z
dc.date.available2024-03-09T18:48:51Z
dc.date.issued2023
dc.departmentİzmir Bakırçay Üniversitesien_US
dc.description.abstractBackground/aim: The subject of this study was to investigate the utility of platelet-rich plasma (PRP) in the cryopreservation process to reduce cryodamage and increase tissue viability.Materials and methods: Twenty-one female Wistar rats were randomly allocated to three groups. In Group 1 (G1), rats were not subjected to vitrification (n = 7). Group 2 (G2) was the vitrification group in which PRP was added to the basic vitrification solution (n = 7). Group 3 (G3) was the vitrification group in which fetal bovine serum was added to the basic vitrification solution (n = 7). Warmed tissues were evaluated with histochemical (HC) and immunohistochemical (IHC) staining, the TUNEL method, immunofluorescence (IF) staining, and biochemical analyses.Results: The percentages of IHC staining, TUNEL method positivity, and IF staining were significantly higher in G2 compared to both G1 and G3 (P < 0.05). G2 ovaries exhibited a significant increase in both malondialdehyde and catalase values in comparison to G1 (P < 0.05). In HC staining, degenerations in primary and secondary follicles and in ovarian tissue were more common in the PRP-supplemented group. The calcium used in PRP activation was suspected to have increased the degeneration and prevented the possible positive effects of PRP.Conclusion: To the best of our knowledge, PRP-supplemented vitrification solution was used for the first time in the literature in this study in whole rat ovarian tissue vitrification. If PRP is to be used as a component in vitrification solution for rat ovarian tissue, the use of lower amounts of calcium or different methods in PRP activation, or the use of nonactivated PRP, should be considered from the beginning.en_US
dc.identifier.doi10.55730/1300-0144.5694
dc.identifier.endpage+en_US
dc.identifier.issn1300-0144
dc.identifier.issn1303-6165
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85175851132en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1281en_US
dc.identifier.trdizinid1209075en_US
dc.identifier.urihttps://doi.org/10.55730/1300-0144.5694
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1209075
dc.identifier.urihttps://hdl.handle.net/20.500.14034/1507
dc.identifier.volume53en_US
dc.identifier.wosWOS:001108669900041en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.publisherTubitak Scientific & Technological Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal of Medical Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCalcium; Cryopreservation; Platelet-Rich Plasma; Ovary; Vitrificationen_US
dc.titleEffects of vitrification solution supplemented with platelet-rich plasma in rat ovarian tissue cryopreservationen_US
dc.typeArticleen_US

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