Impact of melamine exposure on apoptotic proteins and oxidative stress markers in mouse hepatic tissue

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dc.contributor.authorDevrim, Alparslan Kadir
dc.contributor.authorDevrim, Tuba
dc.contributor.authorEkici, Hüsamettin
dc.contributor.authorSözmen, Mahmut
dc.contributor.authorŞenol, Ali
dc.contributor.authorKuruca, Nilüfer
dc.contributor.authorSudağıdan, Mert
dc.date.accessioned2025-03-21T07:37:59Z
dc.date.available2025-03-21T07:37:59Z
dc.date.issued2025
dc.departmentİzmir Bakırçay Üniversitesi
dc.descriptionProject number 2019/053.
dc.description.abstractMost melamine studies have focused on renal toxicity and its effects on the liver are still not well known. We investigated the apoptotic and oxidative effects of melamine on the liver using thirty BALB/c mice, divided into three groups. The control group received saline, while the low-dose melamine (LDM) group was given 400 mg/kg (1/8 LD50) and the high-dose melamine (HDM) group received 1600 mg/kg (1/2 LD50) intragastrically (0.Most melamine studies have focused on renal toxicity and its effects on the liver are still not well known. We investigated the apoptotic and oxidative effects of melamine on the liver using thirty BALB/c mice, divided into three groups. The control group received saline, while the low-dose melamine (LDM) group was given 400 mg/kg (1/8 LD50) and the high-dose melamine (HDM) group received 1600 mg/kg (1/2 LD50) intragastrically (0.25 ml) for 5 consecutive days. Liver Bcl-2 and caspase-3 expressions were analyzed at the protein level by immunohistochemistry and ELISA, and also at the gene level by quantitative Real-Time PCR. In addition, total antioxidant (TAS), total oxidant (TOS), and oxidative stress index (OSI) levels in liver tissues were measured spectrophotometrically. The immunohistochemical expression of caspase-3 was higher in the LDM and HDM groups compared to the control group (p = 0.002). TOS and OSI levels were increased significantly (P ml) for 5 consecutive days. Liver Bcl-2 and caspase-3 expressions were analyzed at the protein level by immunohistochemistry and ELISA, and also at the gene level by quantitative Real-Time PCR. In addition, total antioxidant (TAS), total oxidant (TOS), and oxidative stress index (OSI) levels in liver tissues were measured spectrophotometrically. The immunohistochemical expression of caspase-3 was higher in the LDM and HDM groups compared to the control group (p = 0.002). TOS and OSI levels were increased significantly (P
dc.description.sponsorshipAnkara University
dc.identifier.doi10.33988/auvfd.1548798
dc.identifier.issn1300-0861
dc.identifier.issn1308-2817
dc.identifier.startpage7-Jan
dc.identifier.urihttps://hdl.handle.net/20.500.14034/2627
dc.identifier.urihttps://doi.org/10.33988/auvfd.1548798
dc.language.isoen
dc.publisherAnkara Üniversitesi
dc.relation.ispartofAnkara Üniversitesi Veteriner Fakültesi Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_DergiPark_20250319
dc.subjectApoptosis
dc.subjectBcl-2
dc.subjectCaspase-3
dc.subjectMelamine
dc.subjectOxidative stress
dc.titleImpact of melamine exposure on apoptotic proteins and oxidative stress markers in mouse hepatic tissue
dc.typeArticle

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