Investigation of the potential effects of alpha-lipoic acid on human degenerated intervertebral disc tissue primary cell cultures

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dc.authoridAYDIN, IRFAN/0000-0001-5488-2262
dc.authoridOzbek, hanefi/0000-0002-8084-7855
dc.authorwosidAYDIN, IRFAN/AAK-9913-2021
dc.authorwosidOzbek, hanefi/O-3472-2019
dc.contributor.authorAydın, İrfan
dc.contributor.authorYaşar Şirin, Duygu
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorÖzbek, Hanefi
dc.date.accessioned2023-03-22T19:47:30Z
dc.date.available2023-03-22T19:47:30Z
dc.date.issued2022
dc.departmentBelirleneceken_US
dc.description.abstractAIM: To investigate the supplementation of alpha-lipoic acid (ALA) at the molecular level to determine its effect on primary cell cultures prepared from human intervertebral disc (IVD) tissue in an in vitro environment. MATERIAL and METHODS: Human primary cell cultures were prepared from IVD tissue resected during surgery. While cell cultures without ALA supplementation formed the control group, those with ALA supplementation formed the study group. All cell groups were stained using acridine orange/propidium iodide (AO/PI), and the incidence of apoptotic cell death was determined under a fluorescent microscope. Cell surface morphology and extracellular matrix (ECM) structures were evaluated under an invert light microscope. Simultaneously, cell proliferation was evaluated by MTT???ELISA analysis, and the expressions of chondroadherin (CHAD), cartilage oligomeric protein (COMP), interleukin-1 beta (IL-1??), and matrix metalloproteinase (MMP)-7 and-19, which are genes associated with ECM regulation, were tested using qRT???PCR. The data obtained were evaluated statistically using Tukey???s honestly significant difference (HSD) test after analysis of variance (ANOVA) was performed. The alpha significance value was accepted as < .05. RESULTS: Compared to the cells in the control group, it was observed that both proliferation was suppressed and ECM structures deteriorated in the cells in the study group. CONCLUSION: Also, it was reported that the all-gene expression levels changed. ALA supplementation can negatively affect human IVD primary cell cultures in an in vitro environment.en_US
dc.identifier.doi10.5137/1019-5149.JTN.34917-21.3
dc.identifier.endpage429en_US
dc.identifier.issn1019-5149
dc.identifier.issue3en_US
dc.identifier.pmid35147963en_US
dc.identifier.scopus2-s2.0-85130493382en_US
dc.identifier.scopusqualityN/Aen_US
dc.identifier.startpage420en_US
dc.identifier.urihttps://doi.org/10.5137/1019-5149.JTN.34917-21.3
dc.identifier.urihttps://hdl.handle.net/20.500.14034/736
dc.identifier.volume32en_US
dc.identifier.wosWOS:000804648400012en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTurkish Neurosurgical Socen_US
dc.relation.journalTurkish Neurosurgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha-lipoic aciden_US
dc.subjectInterleukin-1 betaen_US
dc.subjectIntervertebral discen_US
dc.subjectCartilage oligomeric matrix proteinen_US
dc.subjectChondroadherin geneen_US
dc.subjectMatrix metalloproteinaseen_US
dc.subjectAcetyl-L-Carnitineen_US
dc.subjectHippocampal Tissueen_US
dc.subjectNucleus Pulposusen_US
dc.subjectPainen_US
dc.subjectProliferationen_US
dc.subjectProteinen_US
dc.subjectGeneen_US
dc.titleInvestigation of the potential effects of alpha-lipoic acid on human degenerated intervertebral disc tissue primary cell culturesen_US
dc.typeArticleen_US

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