Investıgatıon of ursodeoxycholıc acıd effects on sırolımus treated adıpose tıssue-derıved mesenchymal stem cells

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dc.contributor.authorUyanıkgil, Yiğit
dc.contributor.authorGündüz, Cumhur
dc.contributor.authorYiğittürk, Gürkan
dc.contributor.authorÇavuşoğlu, Türker
dc.contributor.authorGökçe, Burak
dc.contributor.authorNone, Senem
dc.contributor.authorTomruk, Canberk
dc.date.accessioned2023-03-22T19:47:58Z
dc.date.available2023-03-22T19:47:58Z
dc.date.issued2022
dc.departmentBelirleneceken_US
dc.description.abstractObjective The usage of mesenchymal stem cells (MSC) with immunosuppressive drugs after organ transplantation is becoming remarkable in clinical applications. However, the drugs negatively affect MSCs. Ursodeoxycholic acid (UDCA), which is an antioxidant molecule, may reverse these effects. The study aims that to determine the effects of sirolimus and UDCA on human adipose tissue-derived MSCs (ADMSCs) individually and in combination. Material and Method The cytotoxicity of the agents was evaluated by WST-1 test in time and dose-dependent manner. The combinational effects were determined using isobologram analysis. Muse cell analyzer was used for the evaluation of apoptosis and cell cycle. Oxidative stress markers were measured by biochemical methods. Results IC50 dose of sirolimus was determined as 18.58?M in the 48th hour. Because no cytotoxic effect was observed at the studied doses of UDCA, the apoptosis, cell cycle, and oxidative stress indicator analyses were continued with a safe dose of 100 ?M. Sirolimus promoted apoptosis and inhibited cell proliferation. It was determined that UDCA reduced the apoptotic and anti-proliferative effects of sirolimus on ADMSCs with its anti-oxidant property. Conclusion The UDCA treatment in combination with immunosuppressive therapy after organ and tissue transplantation may have positive effects on ADMSCs.en_US
dc.identifier.doi10.17343/sdutfd.1077520
dc.identifier.endpage334en_US
dc.identifier.issn1300-7416
dc.identifier.issn2602-2109
dc.identifier.issue3en_US
dc.identifier.startpage324en_US
dc.identifier.trdizinid1128365en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14034/931
dc.identifier.urihttps://doi.org/10.17343/sdutfd.1077520
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1128365
dc.identifier.volume29en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.journalSüleyman Demirel Üniversitesi Tıp Fakültesi Dergisien_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSirolimusen_US
dc.subjectUrsodeoxycholic aciden_US
dc.subjectOxidative stressen_US
dc.subjectHuman adipose tissue-derived mesenchymal stem cellsen_US
dc.titleInvestıgatıon of ursodeoxycholıc acıd effects on sırolımus treated adıpose tıssue-derıved mesenchymal stem cellsen_US
dc.typeArticleen_US

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