Comparison of broth microdilution method with BD phoenix, micro scan and e-test for carbapenem-resistant enterobacterales colistin susceptibility testing

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dc.contributor.authorYiş, Reyhan
dc.date.accessioned2023-03-22T19:48:10Z
dc.date.available2023-03-22T19:48:10Z
dc.date.issued2022
dc.departmentBelirleneceken_US
dc.description.abstractObjective: In the past years, due to the increasing carbapenem resistant Enterobacterales (CRE) infection rates, colistin use has been on the rise. Multi-drug resistant Gram-negative bacteria and colistin resistance are increasing simultaneously; therefore, an accurate method for antimicrobial susceptibility testing of colistin is crucial. Clinical and Laboratory Standards Institute and European Committee on Antimicrobial Susceptibility Testing recommend the standard broth microdilution (BMD) method for colistin minimum inhibitory concentration testing. In this study, we aimed to examine the performance of BD Phoenix, MicroScan, and E-tests on CRE isolates on the determination of colistin susceptibility. The existing commercial tests were compared to the reference BMD method. Methods: One hundred and twenty non-duplicate clinical Enterobacterales isolates such as Klebsiella pneumoniae (K. pneumoniae), Escherichia coli (E. coli), Enterobacter cloace (E. cloacae) were collected between August 2017 to June 2018. The BD Phoenix, MicroScan systems, and E-tests were used to test colistin susceptibility. Commercial methods were compared with the reference method BMD. Results: Colistin susceptibility was evaluated in 120 Gram-negative clinical isolates, including 108 K. pneumoniae, 10 E. coli, 2 E. cloacae, during the study period. Among the isolates, 66 (55%) were susceptible, and 54 (45%) were resistant to colistin, according to BMD. BD Phoenix, MicroScan, and E-test had 90.90%, 95.45%, and 96.96% sensitivity, respectively, when colistin was tested. Conclusion: In routine clinical practice, the worldwide reference method can hardly be implemented, and commercially available systems are used for the interpretation of colistin susceptibility. Colistin use is increasing for the treatment of multiresistant Gram-negative infections, further and more extensive studies are needed for precise susceptibility testing methods for this compound. We recommend that laboratories use the BMD method at least in selected patient groups in the face of increasing antimicrobial resistance.en_US
dc.identifier.doi10.4274/jarem.galenos.2022.7029
dc.identifier.endpage65en_US
dc.identifier.issn2146-6505
dc.identifier.issn2147-1894
dc.identifier.issue2en_US
dc.identifier.startpage61en_US
dc.identifier.trdizinid1128390en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14034/1003
dc.identifier.urihttps://doi.org/10.4274/jarem.galenos.2022.7029
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1128390
dc.identifier.volume12en_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.journalJAREMen_US
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleComparison of broth microdilution method with BD phoenix, micro scan and e-test for carbapenem-resistant enterobacterales colistin susceptibility testingen_US
dc.typeArticleen_US

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