Investigation of the relationship between inflammation and microbiota in the intestinal tissue of female and male rats fed with fructose: Modulatory role of metformin

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dc.authoridYalcin Bugdayci, Azimet/0000-0003-0923-2097
dc.contributor.authorBugdayci, Azimet Yalcin
dc.contributor.authorDizakar, Saadet Ozen Akarca
dc.contributor.authorDemirel, Muerside Ayse
dc.contributor.authorOmeroglu, Suna
dc.contributor.authorAkar, Fatma
dc.contributor.authorUludag, Mecit Orhan
dc.date.accessioned2025-03-20T09:51:14Z
dc.date.available2025-03-20T09:51:14Z
dc.date.issued2024
dc.departmentİzmir Bakırçay Üniversitesi
dc.description.abstractBackground It has been reported that High-Fructose (HF) consumption, considered one of the etiological factors of Metabolic Syndrome (MetS), causes changes in the gut microbiota and metabolic disorders. There is limited knowledge on the effects of metformin in HF-induced intestinal irregularities in male and female rats with MetS. Objectives In this study, we investigated the sex-dependent effects of metformin treatment on the gut microbiota, intestinal Tight Junction (TJ) proteins, and inflammation parameters in HF-induced MetS. Methods Fructose was given to the male and female rats as a 20% solution in drinking water for 15 weeks. Metformin (200 mg/kg) was administered by gastric tube once a day during the final seven weeks. Biochemical, histopathological, immunohistochemical, and bioinformatics analyses were performed. Differences were considered statistically significant at p < 0.05. Results The metformin treatment in fructose-fed rats promoted glucose, insulin, Homeostasis Model Assessment of Insulin Resistance Index (HOMA-IR), and Triglyceride (TG) values in both sexes. The inflammation score was significantly decreased with metformin treatment in fructose-fed male and female rats (p < 0.05). Moreover, metformin treatment significantly decreased Interleukin-1 Beta (IL-1 beta) and Tumor Necrosis Factor-Alpha (TNF-alpha) in ileum tissue from fructose-fed males (p < 0.05). Intestinal immunoreactivity of Occludin and Claudin-1 was increased with metformin treatment in fructose-fed female rats. HF and metformin treatment changed the gut microbial composition. Firmicutes/Bacteroidetes (F/B) ratio increased with HF in females. In the disease group, Bifidobacterium pseudolongum; in the treatment group, Lactobacillus helveticus and Lactobacillus reuteri are the prominent species in both sexes. When the male and female groups were compared, Akkermansia muciniphila was prominent in the male treatment group. Conclusion In conclusion, metformin treatment promoted biochemical parameters in both sexes of fructose-fed rats. Metformin showed a sex-dependent effect on inflammation parameters, permeability factors, and gut microbiota. Metformin has partly modulatory effects on fructose-induced intestinal changes.
dc.description.sponsorshipScientific and Technological Research Council of Turkiye (TUBITAK); Gazi University Scientific Research Project Commission [Gazi BAP-5937, 02/2019-06]
dc.description.sponsorshipOpen access funding provided by the Scientific and Technological Research Council of Turkiye (TUBITAK). This study was produced from the Ph. D. Thesis prepared by the first author under the supervision of the sixth author. This study was supported by Gazi University Scientific Research Project Commission with project number Gazi BAP-5937 and project code 02/2019-06.
dc.identifier.doi10.1007/s40199-024-00521-2
dc.identifier.endpage535
dc.identifier.issn2008-2231
dc.identifier.issue2
dc.identifier.pmid38884844
dc.identifier.scopus2-s2.0-85196106304
dc.identifier.scopusqualityQ1
dc.identifier.startpage515
dc.identifier.urihttps://doi.org/10.1007/s40199-024-00521-2
dc.identifier.urihttps://hdl.handle.net/20.500.14034/2476
dc.identifier.volume32
dc.identifier.wosWOS:001249564400001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Int Publ Ag
dc.relation.ispartofDaru-Journal of Pharmaceutical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250319
dc.subjectHigh-fructose diet
dc.subjectGut microbiota
dc.subjectMetformin
dc.subjectMetabolic syndrome
dc.subjectInflammation
dc.subjectGut permeability
dc.titleInvestigation of the relationship between inflammation and microbiota in the intestinal tissue of female and male rats fed with fructose: Modulatory role of metformin
dc.typeArticle

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