Platelet-Rich Plasma in Vitrification; is it Helpful or Harmful?

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dc.authoridTomruk, Canberk/0000-0002-3810-3705
dc.authoridSirin, Cansin/0000-0002-4530-701X
dc.authoridGökhan, Aylin/0000-0002-6254-157X
dc.authorwosidTomruk, Canberk/AAN-4976-2020
dc.authorwosidSirin, Cansin/ABI-3816-2020
dc.authorwosidKilic, Kubilay Dogan/GSJ-0645-2022
dc.authorwosidGökhan, Aylin/AAE-2494-2019
dc.contributor.authorCavusoglu, Turker
dc.contributor.authorGokhan, Aylin
dc.contributor.authorTomruk, Canberk
dc.contributor.authorSirin, Cansin
dc.contributor.authorKilic, Kubilay Dogan
dc.contributor.authorYigitturk, Guerkan
dc.date.accessioned2024-03-09T18:48:45Z
dc.date.available2024-03-09T18:48:45Z
dc.date.issued2023
dc.departmentİzmir Bakırçay Üniversitesien_US
dc.description.abstractHuman and animal studies on cryoprotectants and freezing solutions are still needed to establish a simple yet reliable protocol and increase the success of cryopreservation. The main aim of this study was to evaluate the short- and long-term effects of platelet-rich plasma, a well-known antioxidant substance due to its contents including bioactive molecules and growth factors, on whole ovarian tissue cryopreservation. Fresh tissues (control group, G1) were subjected to histological tissue processing without any treatment. Ovaries treated with platelet-rich plasma (PRP)-supplemented vitrification solution were subjected to tissue processing without cryostorage group 2 (G2) or following six months of cryostorage group 3 (G3). Steps in G2 and G3 were also performed for group 4 (G4) and group 5 (G5), respectively, except that the vitrification solution was supplemented with fetal bovine serum. PRP was activated with calcium chloride (CaCl2) after double centrifugation. Ethylene glycol, dimethyl sulfoxide, and sucrose were used as cryoprotective agents in all groups. Histomorphological changes were evaluated with the semi-quantitative histochemical-scoring algorithm. Apoptotic and antiapoptotic effects and intercellular connections were evaluated with immunohistochemical staining of Bax, Bcl-2, Caspase-3 (C3), Connexin-43 (Cx-43), and TUNEL analysis. Cryopreservation with PRP-supplementation (G3) significantly increased tissue degeneration (p<0.05). There was an increase in the number of degenerated both primary and secondary follicles (p<0.05), and an increase in the immune expression of Bax, C3 and Cx-43 and TUNEL assay in G3 was observed compared to other groups (p<0.05). Since the morphology of primordial follicles was more preserved than other follicles in all groups, primordial follicles were not included in the follicle count. Our study suggested that cryopreservation with PRP-supplemented vitrification solution caused excessive damage to rat ovaries. We assumed that CaCl2 might have further provoked this cellular damage.en_US
dc.description.sponsorshipEge University Scientific Research Projects Coordination Unit [TKB-2019-20518]en_US
dc.description.sponsorshipFunding Statement: This study is supported by Ege University Scientific Research Projects Coordination Unit (Project Number: TKB-2019-20518) .en_US
dc.identifier.doi10.29261/pakvetj/2023.077
dc.identifier.endpage506en_US
dc.identifier.issn0253-8318
dc.identifier.issn2074-7764
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85173947039en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage500en_US
dc.identifier.urihttps://doi.org/10.29261/pakvetj/2023.077
dc.identifier.urihttps://hdl.handle.net/20.500.14034/1452
dc.identifier.volume43en_US
dc.identifier.wosWOS:001085009600017en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherUniv Agriculture, Fac Veterinary Scienceen_US
dc.relation.ispartofPakistan Veterinary Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPlatelet-Rich Plasma; Vitrification; Cryopreservation; Calcium; Ethylene Glycol; Dimethyl Sulfoxideen_US
dc.titlePlatelet-Rich Plasma in Vitrification; is it Helpful or Harmful?en_US
dc.typeArticleen_US

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