Effects of Tocilizumab on Intervertebral Disc Degeneration, Cell Senescence and Inflammation via BMP-2, Hif-1α, IL-1β and SOX9

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dc.authoridYILMAZ, Ibrahim/0000-0003-2003-6337
dc.contributor.authorYilmaz, Ibrahim
dc.contributor.authorAkalan, Hande
dc.contributor.authorSirin, Duygu Yasar
dc.contributor.authorKaraarslan, Numan
dc.contributor.authorKasim, Emin
dc.contributor.authorOzbek, Hanefi
dc.contributor.authorAtes, Ozkan
dc.date.accessioned2025-03-20T09:50:29Z
dc.date.available2025-03-20T09:50:29Z
dc.date.issued2023
dc.departmentİzmir Bakırçay Üniversitesi
dc.description.abstractBackground and Objective: Immunosuppressive tocilizumab (TCZ), which is frequently used in the treatment of rheumatoid arthritis, can have many side effects as well as an uncontrolled inflammatory response. This study aimed to evaluate the effect of tocilizumab (TCZ) administered to intervertebral disc (IVD) tissues in vitro on the proinflammatory cytokines and proteins of degeneration, senescence and inflammation-related signaling pathways at the pharmaco-molecular level. Materials and Methods: Primary cell cultures were prepared using human IVD tissues obtained during lumbar microdiscectomy. Untreated groups served as the control and TCZ-treated groups as the study sample. Analyses were performed using a commercial kit, supravital and fluorescent dyes. Changes in bone morphogenetic protein (BMP)-2, hypoxia-inducible factor (Hif)1-alpha (Hif1-alpha), interleukin (IL)-1 beta (IL-1 beta) and sex-determining region Y (SRY)-box 9 (SOX9) protein expressions were evaluated using western blotting. An alpha value of less than 0.05 was considered significant. Results: Proliferation decreased in the samples treated with TCZ (10 mu g mLG1 ) on day 15 (p<0.05). Protein expressions of BMP-2, Hif-1 alpha, IL-1 beta and SOX9, which play a vital role in anabolic and catabolic pathways, changed in samples treated with TCZ (10 mu g mLG1 ). Conclusion: This change was statistically significant (p<0.05). Therefore, results concluded that the inflammation, extracellular matrix degradation and nucleus pulposus degeneration after disc herniation are controlled by BMP-2, Hif-1 alpha, IL-1 beta and SOX9.
dc.identifier.doi10.3923/ijp.2023.825.833
dc.identifier.issn1811-7775
dc.identifier.issn1812-5700
dc.identifier.issue6
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://doi.org/10.3923/ijp.2023.825.833
dc.identifier.urihttps://hdl.handle.net/20.500.14034/2220
dc.identifier.volume19
dc.identifier.wosWOS:001171399400003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherAsian Network Scientific Information-Ansinet
dc.relation.ispartofInternational Journal of Pharmacology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250319
dc.subjectBMP-2
dc.subjectHif-1 alpha
dc.subjectIL-1 beta
dc.subjectSOX9
dc.subjecttocilizumab
dc.subjectdisc herniation
dc.subjectproinflammatory cytokines
dc.subjectoral herpes simplex
dc.subjectmorphogenetic protein-2
dc.titleEffects of Tocilizumab on Intervertebral Disc Degeneration, Cell Senescence and Inflammation via BMP-2, Hif-1α, IL-1β and SOX9
dc.typeArticle

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