Development of VEGF mimetic QK peptide on polycaprolactone nanofiber for vascular tissue engineering

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dc.authoridYARALI CEVIK, ZIYSAN BUSE/0000-0002-9371-6424
dc.contributor.authorCevik, Ziysan Buse Yarali
dc.contributor.authorAhsunal, Gulsah
dc.contributor.authorTaskara, Aytan
dc.contributor.authorKaraman, Ozan
dc.date.accessioned2025-03-20T09:51:08Z
dc.date.available2025-03-20T09:51:08Z
dc.date.issued2024
dc.departmentİzmir Bakırçay Üniversitesi
dc.description.abstractElectrospinning allows promoting vascular tissue formation. Polycaprolactone (PCL) is a promising polymer for vascular tissue engineering because of its great mechanical strength and efficient fabrication. However, PCL shows limited biocompatibility owing to its hydrophobic structure. PCL nanofibers can be modified by peptides to improve biological activity. KLTWQELYQLKYKGI (QK) is a peptide that mimics the Vascular Endothelial Growth Factor (VEGF) by organizing endothelial cells. It is critically important to identify the optimum concentration of QK peptide conjugation onto PCL nanofiber for vascularization. Thus, PCL nanofibers were conjugated by different QK peptide concentrations (10 mu M, 100 mu M and 1000 mu M). QK conjugated nanofibers were characterized by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) analysis. The vascularization was evaluated by Live and Dead staining assay, real time PCR of platelet/endothelial cell adhesion molecule (PECAM-1), VEGF and vascular endothelial-cadherin (VE-cadherin) genes and VEGF immunofluorescence analysis. The surfaces of PCL nanofibers were modified by different concentrations of QK peptide. The roughness of nanofibers increased depending on the increased concentration of QK peptide. The higher cell attachment and cell viability were observed in 1000 mu M of QK peptide. 1000 mu M of QK peptide showed higher vasculogenic potential in terms of PECAM, VEGF, and VE-cadherin gene expression than all the other experimental groups. In the same on gene expression analysis by qPCR, 1000 mu M concentration of QK peptide showed the higher VEGF protein than the other experimental groups. 1000 mu M concentration of QK peptide can be used to improve the vasculogenic property of PCL nanofibers.
dc.identifier.doi10.1016/j.mtcomm.2024.109026
dc.identifier.issn2352-4928
dc.identifier.scopus2-s2.0-85191488081
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.mtcomm.2024.109026
dc.identifier.urihttps://hdl.handle.net/20.500.14034/2434
dc.identifier.volume39
dc.identifier.wosWOS:001237574800001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofMaterials Today Communications
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250319
dc.subjectPolycaprolactone (PCL)
dc.subjectNanofiber
dc.subjectQK peptide
dc.subjectVasculogenesis
dc.titleDevelopment of VEGF mimetic QK peptide on polycaprolactone nanofiber for vascular tissue engineering
dc.typeArticle

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