Assessing effects of caffeic acid on cytotoxicity, apoptosis, invasion, GST enzyme activity, oxidant, antioxidant status and micro-RNA expressions in HCT116 colorectal cancer cells

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dc.authoridMutlu, Dogukan/0000-0003-3259-5822
dc.authorwosidMutlu, Dogukan/AAL-4976-2021
dc.contributor.authorSecme, Mucahit
dc.contributor.authorMutlu, Dogukan
dc.contributor.authorElmas, Levent
dc.contributor.authorArslan, Sevki
dc.date.accessioned2024-03-09T18:48:29Z
dc.date.available2024-03-09T18:48:29Z
dc.date.issued2023
dc.departmentİzmir Bakırçay Üniversitesien_US
dc.description.abstractIn developed western countries, colorectal cancer is one of the most prevalent cancer types. Natural bioactive compounds attract attention due to their antioxidant, anti-inflammatory, anticancer and therapeutic activities. Caffeic acid (3,4-dihydroxycinnamic) is one of the one of the most common phenolic acids found in many foods and plants such as coffee, honey, blueberries, herbs, potatoes, apples. The aim of the study is investigating cytotoxic, apoptotic, anti-invasive, oxidant, antioxidant, GST inhibitory activities of caffeic acid in colon cancer cells. Another aim of this study is to demonstrate a mechanistic approach by investigating the expression changes of mRNA and micro RNAs that play a role in these cellular mechanisms. In this context, MTT, Annexin-V, Matrigel invasion, TAS-TOS, GST enzyme activity assay and Real-Time PCR experiments were performed under in vitro conditions. According to results, caffeic acid have exhibits antiproliferative, apoptotic and anti-invasive activity in HCT116 cells. It caused changes expression of apoptosis, cell cycle and invasion-related genes and microRNAs. Caffeic acid also reduced oxidative stress and GST enzyme activity. Taken together, all these results reveal that caffeic acid may be a therapeutic agent in HCT116 colorectal cancer cells. This study will shed light on future comprehensive studies. (c) 2023 SAAB. Published by Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.sajb.2023.03.046
dc.identifier.endpage26en_US
dc.identifier.issn0254-6299
dc.identifier.issn1727-9321
dc.identifier.scopus2-s2.0-85151477967en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage19en_US
dc.identifier.urihttps://doi.org/10.1016/j.sajb.2023.03.046
dc.identifier.urihttps://hdl.handle.net/20.500.14034/1337
dc.identifier.volume157en_US
dc.identifier.wosWOS:000964321800001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofSouth African Journal of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaffeic Acid; Colorectal Cancer; Hct116 Cells; Anticancer Activityen_US
dc.titleAssessing effects of caffeic acid on cytotoxicity, apoptosis, invasion, GST enzyme activity, oxidant, antioxidant status and micro-RNA expressions in HCT116 colorectal cancer cellsen_US
dc.typeArticleen_US

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