The effects of PKI-402 on breast tumor models' radiosensitivity via dual inhibition of PI3K/mTOR

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dc.authoridGunduz, Cumhur/0000-0002-6593-3237
dc.authoridYILMAZ SUSLUER, SUNDE/0000-0002-0535-150X
dc.authoridAŞIK, Aycan/0000-0002-4123-4175
dc.authoridBiray Avci, Cigir/0000-0001-8251-4520
dc.authoridsogutlu, fatma/0000-0002-1210-7660
dc.authoridGASIMLI, Roya/0000-0002-6760-8921
dc.authorwosidGunduz, Cumhur/C-1243-2014
dc.authorwosidYILMAZ SUSLUER, SUNDE/HJZ-2716-2023
dc.authorwosidAŞIK, Aycan/A-8211-2019
dc.authorwosidBiray Avci, Cigir/AAH-2300-2019
dc.authorwosidsogutlu, fatma/ABE-2054-2020
dc.contributor.authorGasimli, Roya
dc.contributor.authorKayabasi, Cagla
dc.contributor.authorYelken, Besra Ozmen
dc.contributor.authorAsik, Aycan
dc.contributor.authorSogutlu, Fatma
dc.contributor.authorCelebi, Caglar
dc.contributor.authorSusluer, Sunde Yilmaz
dc.date.accessioned2024-03-09T18:48:30Z
dc.date.available2024-03-09T18:48:30Z
dc.date.issued2023
dc.departmentİzmir Bakırçay Üniversitesien_US
dc.description.abstractPurposePI3K/Akt/mTOR pathway activation causes relapse and resistance after radiotherapy in breast cancer (BC). We aimed to radiosensitize BC cell lines to irradiation (IR) by PKI-402, a dual PI3K/mTOR inhibitor.MethodsWe performed cytotoxicity, clonogenicity, hanging drop, apoptosis and double-strand break detection, and phosphorylation of 16 essential proteins involved in the PI3K/mTOR pathway.ResultsOur findings showed that PKI-402 has cytotoxic efficiency in all cell lines. Clonogenic assay results showed that PKI-402 plus IR inhibited the colony formation ability of MCF-7 and breast cancer stem cell lines. Results showed that PKI-402 plus IR causes more apoptotic cell death than IR alone in the MCF-7 cells but did not cause significant changes in the MDA-MB-231. & gamma;-H2AX levels were increased in MDA-MB-231 in PKI-402 plus IR groups, whereas we did not observe any apoptotic and & gamma;-H2AX induction in BCSCs and MCF-10A cells in all treatment groups. Some pivotal phosphorylated proteins of the PI3K/AKT pathway decreased, several proteins increased and others did not change.ConclusionIn conclusion, if the combined use of PKI-402 with radiation is supported by in vivo studies, it can contribute to the treatment options and the course of the disease.en_US
dc.description.sponsorshipEge University Scientific Research Projects (BAP) Department [2017-TIP-022, 18-KSUAM-001]en_US
dc.description.sponsorshipThis study was supported by Ege University Scientific Research Projects (BAP) Department (Grant No. 2017-TIP-022, 18-KSUAM-001).en_US
dc.identifier.doi10.1080/09553002.2023.2232019
dc.identifier.endpage1970en_US
dc.identifier.issn0955-3002
dc.identifier.issn1362-3095
dc.identifier.issue12en_US
dc.identifier.pmid37389464en_US
dc.identifier.scopus2-s2.0-85165130811en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1961en_US
dc.identifier.urihttps://doi.org/10.1080/09553002.2023.2232019
dc.identifier.urihttps://hdl.handle.net/20.500.14034/1352
dc.identifier.volume99en_US
dc.identifier.wosWOS:001026779100001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofInternational Journal of Radiation Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBreast Cancer; Radiosensitivity; Pi3k; Mtor Pathway; Pki-402; >en_US
dc.titleThe effects of PKI-402 on breast tumor models' radiosensitivity via dual inhibition of PI3K/mTORen_US
dc.typeArticleen_US

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