Monensin, an Antibiotic Isolated from Streptomyces Cinnamonensis, Regulates Human Neuroblastoma Cell Proliferation via the PI3K/AKT Signaling Pathway and Acts Synergistically with Rapamycin

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dc.authoridSerter Koçoğlu, Sema/0000-0002-3180-4007;
dc.authorwosidSerter Koçoğlu, Sema/ABQ-8779-2022
dc.authorwosidKORKUSUZ, Gozde/JVZ-5576-2024
dc.contributor.authorKocoglu, Sema Serter
dc.contributor.authorSecme, Muecahit
dc.contributor.authorOy, Ceren
dc.contributor.authorKorkusuz, Gozde
dc.contributor.authorElmas, Levent
dc.date.accessioned2024-03-09T18:48:46Z
dc.date.available2024-03-09T18:48:46Z
dc.date.issued2023
dc.departmentİzmir Bakırçay Üniversitesien_US
dc.description.abstractNeuroblastoma is the most common extracranial childhood tumor and accounts for approximately 15% of pediatric cancer-related deaths. Further studies are needed to identify potential therapeutic targets for neuroblastoma. Monensin is an ionophore antibiotic obtained from Streptomyces cinnamonensis with known antibacterial and antiparasitic effects. No study has reported the effects of monensin on SH-SY5Y neuroblastoma cells by targeting the PI3K/AKT signaling pathway. The aim of this study was to investigate the antiproliferative effects of monensin alone and in combination with rapamycin in human SH-SY5Y neuroblastoma cells mediated by the PI3K/AKT signaling pathway. The effects of single and combination applications of monensin and rapamycin on SH-SY5Y cell proliferation were investigated by XTT, and their effects on the PI3K/AKT signaling pathway by RT-PCR, immunohistochemistry, immunofluorescence, and Western blotting. The combined effects of monensin and rapamycin on SH-SY5Y proliferation were most potent at 72 h (combination index < 1). The combination of monensin and rapamycin caused a significant decrease in the expression of P21RAS, AKT, and MAPK1 genes. Single and combined administrations of monensin and rapamycin caused a significant decrease in PI3K/AKT expression. Our results showed for the first time that monensin exerts an antiproliferative effect by targeting the PI3K/AKT signaling pathway in neuroblastoma cells. It is suggested that monensin and its combination with rapamycin may be an effective therapeutic candidate for treating neuroblastoma.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey [TUBITAK-SBAG-120S399]en_US
dc.description.sponsorshipThis study was supported by a grant from the Scientific and Technological Research Council of Turkey (TUBITAK-SBAG-120S399).en_US
dc.identifier.doi10.3390/antibiotics12030546
dc.identifier.issn2079-6382
dc.identifier.issue3en_US
dc.identifier.pmid36978413en_US
dc.identifier.scopus2-s2.0-85151363382en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.3390/antibiotics12030546
dc.identifier.urihttps://hdl.handle.net/20.500.14034/1471
dc.identifier.volume12en_US
dc.identifier.wosWOS:000953881400001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofAntibiotics-Baselen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectStreptomyces Cinnamonensis; Monensin; Rapamycin; Neuroblastoma; Anticancer; Pi3k; Akt Pathwayen_US
dc.titleMonensin, an Antibiotic Isolated from Streptomyces Cinnamonensis, Regulates Human Neuroblastoma Cell Proliferation via the PI3K/AKT Signaling Pathway and Acts Synergistically with Rapamycinen_US
dc.typeArticleen_US

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