Development of a novel laboratory photodynamic therapy device: automated multi-mode LED system for optimum well-plate irradiation

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dc.authoridguney eskiler, gamze/0000-0002-2088-9914
dc.authoridTabakoglu, Hasim Ozgur/0000-0001-7239-9856
dc.authoridPALA, Muhammed Ali/0000-0002-8153-7971
dc.contributor.authorYildiz, Mustafa Zahid
dc.contributor.authorKamanli, Ali Furkan
dc.contributor.authorEskiler, Gamze Gueney
dc.contributor.authorTabakoglu, Hasim Ozgur
dc.contributor.authorPala, Muhammed Ali
dc.contributor.authorOzdemir, Ayla Eren
dc.date.accessioned2025-03-20T09:51:20Z
dc.date.available2025-03-20T09:51:20Z
dc.date.issued2024
dc.departmentİzmir Bakırçay Üniversitesi
dc.description.abstractMulti-mode Automated Well-plate PDT LED Laboratory Irradiation System described.Automates and standardizes time-consuming experiments.LED wavelength and temperature stabilized for highly reproducible irradiations.Efficacy demonstrated in 5-aminolevulinic acid (5-ALA) treatments of HT-29 colon cancer cells and WI-38 human lung fibroblasts. Photodynamic therapy (PDT) is a targeted treatment method that utilizes a photosensitizer (PS) to induce cytotoxicity in malignant and non-malignant tumors. Optimization of PDT requires investigation of the selectivity of PS for the target tissues, irradiating light source, irradiation wavelengths, fluence rate, fluence, illumination mode, and overall treatment plan. In this study, we developed the Multi-mode Automatized Well-plate PDT LED Laboratory Irradiation System (MAWPLIS), an innovative device that automates time-consuming well plate light dosage/PS dose measurement experiment. The careful control of LED current and temperature stabilization in the LED module allowed the system to achieve high optical output stability. The MAWPLIS was designed by integrating a 3-axis moving system and motion controller, a quick-switching LED controller unit equipped with interchangeable LED modules capable of employing multiple wavelengths, and a TEC system. The proposed system achieved high optical output stability (1 mW) within the range of 0-500 mW, high wavelength stability (5 nm) at 635 nm, and high temperature stability (0.2 degrees C) across all radiation modes. The system's validation involved in vitro analysis using 5-ALA across varying concentrations, incubation periods, light exposures, and wavelengths in HT-29 colon cancer and WI-38 human lung fibroblast cell lines. Specifically, a combination of 405 nm and 635 nm wavelengths was selected to demonstrate enhanced strategies for colon cancer cell eradication and system validation. The MAWPLIS system represents a significant advancement in photodynamic therapy (PDT) research, offering automation and standardization of time-intensive experiments, high stability and precision, and improved PDT efficacy through dual-wavelength integration.
dc.description.sponsorshipSakarya University of Applied Sciences [118E235]; TUBITAK; Biomedical Technologies Application and Research Center (BIYOTAM)
dc.description.sponsorshipThis study was supported by TUBITAK 3501 Project no: 118E235 and Biomedical Technologies Application and Research Center (BIYOTAM). Special thanks to the Muhammed GULER for support.
dc.identifier.doi10.1007/s10103-024-04083-2
dc.identifier.issn0268-8921
dc.identifier.issn1435-604X
dc.identifier.issue1
dc.identifier.pmid38750381
dc.identifier.scopus2-s2.0-85193359608
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s10103-024-04083-2
dc.identifier.urihttps://hdl.handle.net/20.500.14034/2492
dc.identifier.volume39
dc.identifier.wosWOS:001224502000001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer London Ltd
dc.relation.ispartofLasers in Medical Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250319
dc.subjectPhotodynamic therapy
dc.subjectAutomated experiment
dc.subjectDosage control
dc.subjectDosimetry
dc.subjectEmbedded systems
dc.titleDevelopment of a novel laboratory photodynamic therapy device: automated multi-mode LED system for optimum well-plate irradiation
dc.typeArticle

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