Impact of cisplatin on Kasumi-1 leukemia cell line: gene expression and DNA damage

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dc.contributor.authorDodurga, Yavuz
dc.contributor.authorSeçme, Mücahit
dc.contributor.authorElmas, Levent
dc.contributor.authorDemirkıran, Nazlı
dc.contributor.authorSağ, Sevda
dc.contributor.authorPala, Ulviye
dc.contributor.authorAkdağ, Zeliha
dc.date.accessioned2025-03-20T09:45:02Z
dc.date.available2025-03-20T09:45:02Z
dc.date.issued2025
dc.departmentİzmir Bakırçay Üniversitesi
dc.description.abstractPurpose: Leukemia is a type of cancer caused by the uncontrolled proliferation of blood cells. The purpose of this study was to investigate the effects of cisplatin (CIS), a chemotherapeutic agent used in the treatment of leukemia, on the Kasumi-1 leukemia cell line. Materials and methods: The study measured the effect of CIS on Kasumi-1 cells by calculating IC50 values for cell viability. The mRNA expression levels of apoptosis and cell cycle-related genes were then assessed using Real-Time PCR. In addition, the effects of CIS on DNA damage were investigated using the comet assay. Results: Significant changes in apoptosis and cell cycle-related genes were observed in CIS-treated groups. These included alterations in the mRNA levels of p53, BCL-2, CHECK 1, CDC25C, CDK 6, URG4/URGCP, GADD45A, CCND1, GADD45G, and ATM genes. Comet analysis confirmed CIS's effects on DNA damage. Conclusion: This study aimed to better understand how CIS affects genetic mechanisms in leukemia cells and provide new insights into leukemia treatment. The findings will help us better understand the role of CIS in leukemia treatment and will serve as a valuable reference for future research. © 2025, Pamukkale University. All rights reserved.
dc.identifier.doi10.31362/patd.1552452
dc.identifier.endpage71
dc.identifier.issn1309-9833
dc.identifier.issue1
dc.identifier.scopus2-s2.0-85214525532
dc.identifier.scopusqualityQ4
dc.identifier.startpage63
dc.identifier.urihttps://doi.org/10.31362/patd.1552452
dc.identifier.urihttps://hdl.handle.net/20.500.14034/2134
dc.identifier.volume18
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPamukkale University
dc.relation.ispartofPamukkale Medical Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250319
dc.subjectacute myeloid leukemia
dc.subjectcisplatin
dc.subjectDNA damage
dc.subjectKasumi-1 cell
dc.subjectLeukemia
dc.titleImpact of cisplatin on Kasumi-1 leukemia cell line: gene expression and DNA damage
dc.title.alternativeSisplatinin Kasumi-1 lösemi hücre hattı üzerindeki etkisi: gen ekspresyonu ve DNA hasarı
dc.typeArticle

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