The methylome of buccal epithelial cells is influenced by age, sex, and physiological properties

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dc.authoridKöseler, Aylin/0000-0003-4832-0436
dc.authoridProtti, Giulia/0000-0002-7144-3285
dc.authoridPellegrini, Matteo/0000-0001-9355-9564
dc.authorwosidKöseler, Aylin/C-5030-2012
dc.contributor.authorProtti, Giulia
dc.contributor.authorRubbi, Liudmilla
dc.contributor.authorGoren, Tarik
dc.contributor.authorSabirli, Ramazan
dc.contributor.authorCivlan, Serkan
dc.contributor.authorKurt, Ozgur
dc.contributor.authorTurkcuer, Ibrahim
dc.date.accessioned2024-03-09T18:48:36Z
dc.date.available2024-03-09T18:48:36Z
dc.date.issued2023
dc.departmentİzmir Bakırçay Üniversitesien_US
dc.description.abstractEpigenetic modifications, particularly DNA methylation, have emerged as regulators of gene expression and are implicated in various biological processes and disease states. Understanding the factors influencing the epigenome is essential for unraveling its complexity. In this study, we aimed to identify how the methylome of buccal epithelial cells, a noninvasive and easily accessible tissue, is associated with demographic and health-related variables commonly used in clinical settings, such as age, sex, blood immune composition, hemoglobin levels, and others. We developed a model to assess the association of multiple factors with the human methylome and identify the genomic loci significantly impacted by each trait. We demonstrated that DNA methylation variation is accurately modeled by several factors. We confirmed the well-known impact of age and sex and unveiled novel clinical factors associated with DNA methylation, such as blood neutrophils, hemoglobin, red blood cell distribution width, high-density lipoprotein cholesterol, and urea. Genomic regions significantly associated with these traits were enriched in relevant transcription factors, drugs, and diseases. Among our findings, we showed that neutrophil-impacted loci were involved in neutrophil functionality and maturation. Similarly, hemoglobin-influenced sites were associated with several diseases, including aplastic anemia, and the genomic loci affected by urea were related to congenital anomalies of the kidney and urinary tract. Our findings contribute to a better understanding of the human methylome plasticity and provide insights into novel factors shaping DNA methylation patterns, highlighting their potential clinical implications as biomarkers and the importance of considering these physiological traits in future medical epigenomic investigations.en_US
dc.identifier.doi10.1152/physiolgenomics.00063.2023
dc.identifier.endpage633en_US
dc.identifier.issn1094-8341
dc.identifier.issn1531-2267
dc.identifier.issue12en_US
dc.identifier.pmid37781740en_US
dc.identifier.scopus2-s2.0-85178499215en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage618en_US
dc.identifier.urihttps://doi.org/10.1152/physiolgenomics.00063.2023
dc.identifier.urihttps://hdl.handle.net/20.500.14034/1406
dc.identifier.volume55en_US
dc.identifier.wosWOS:001108027600002en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAmer Physiological Socen_US
dc.relation.ispartofPhysiological Genomicsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBuccal Epithelial Cells; Dna Methylation; Epigenetics; Targeted Bisulfite Sequencingen_US
dc.titleThe methylome of buccal epithelial cells is influenced by age, sex, and physiological propertiesen_US
dc.typeArticleen_US

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