Investigation of oxidative damage, antioxidant balance, DNA repair genes, and apoptosis due to radiofrequency-induced adaptive response in mice

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dc.authorscopusid26534334300
dc.authorscopusid24832794000
dc.authorscopusid57781209000
dc.authorscopusid57885779600
dc.authorscopusid6603181563
dc.contributor.authorKüçükbağrıaçık Yusuf
dc.contributor.authorDastouri Mohammadreza
dc.contributor.authorÖzgür-Büyükkatalay Elçin
dc.contributor.authorAkarca- Dizakar,Saadet Özen
dc.contributor.authorYegin K.
dc.date.accessioned2023-03-22T19:47:49Z
dc.date.available2023-03-22T19:47:49Z
dc.date.issued2022
dc.departmentBelirleneceken_US
dc.description.abstractThis study aims to determine whether exposure to non-ionizing radiofrequency fields could induce an adaptive response (AR) in adult mice and to reveal potential molecular mechanisms triggered by RF-induced AR. The study was performed on 24 adult male Swiss-Albino mice. The average mass of the mice was 37 g. Four groups of adult mice, each consisting of 6, were formed. The radiofrequency group (R) and the adaptive response group (RB) were exposed to 900 MHz of global system for mobile communications (GSM) signal at 0.339 W/kg (1 g average specific absorption rate) 4 h/day for 7 days, while the control group (C) and the bleomycin group (B) were not exposed. 20 minutes after the last radiofrequency field (RF) exposure, the mice in the B and RB groups were injected intraperitoneal (ip) bleomycin (BLM), 37.5 mg/kg. All the animals were sacrificed 30 minutes after the BLM injection. Oxidative damage and antioxidant mechanism were subsequently investigated in the blood samples. Changes in the expression of the genes involved in DNA repair were detected in the liver tissue. TUNEL method was used to determine the apoptosis developed by DNA fragmentation in the liver tissue. The RB group, which produced an adaptive response, was compared with the control group. According to the results, the increase of reactive oxygen species (ROS) in the RB group may have played an important role in triggering the adaptive response and producing the required minimum stress level. Furthermore, tumor suppressor 53(p53), oxo guanine DNA glycosylase (OGG-1) levels responsible for DNA repair mechanism genes expression were increased in conjunction with the increase in ROS. The change in the poly (ADP-ribose) polymerase 1 (PARP-1) and glutathione peroxidase 1 (GPx-1) gene expression were not statistically significant. The antioxidant enzyme levels of superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (TAC) were decreased in the group with adaptive response. According to the data obtained from terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) analysis, apoptosis was decreased in the RB group due to the decrease in cell death, which might have resulted from an increase in gene expression responsible for DNA repair mechanisms. The results of our study show that exposure to RF radiation may create a protective reaction against the bleomycin. The minimal oxidative stress due to the RF exposure leads to an adaptive response in the genes that play a role in the DNA repair mechanism and enzymes, enabling the survival of the cell. © 2022 Taylor & Francis Group, LLC.en_US
dc.description.sponsorship01/2003-62; Ankara Universitesi; Gazi Üniversitesi: 01/2019-47en_US
dc.description.sponsorshipThis research composed with the data of Yusuf Kucukbagriacik’s PhD thesis under the supervision of Dr. Elcin Ozgur Buyukatalay as principal advisor and Dr. Mohammadreza Dastouri as assistant supervisor. The authors would like to thank Dr. Zubeyir Elmazoglu for his technical support. RF-EMF applications and measurements were performed with the devices granted by Gazi University Research Foundation, Project No. 01/2003-62 and 01/2003–62. Biochemical, histological, and genetic analyzes were performed at the Biotechnology Institute of Ankara University and the Faculty of Medicine of Gazi University.en_US
dc.description.sponsorshipThis work was supported by the Gazi University Projects of Scientific Investigation [01/2019-47]. This research composed with the data of Yusuf Kucukbagriacik’s PhD thesis under the supervision of Dr. Elcin Ozgur Buyukatalay as principal advisor and Dr. Mohammadreza Dastouri as assistant supervisor. The authors would like to thank Dr. Zubeyir Elmazoglu for his technical support. RF-EMF applications and measurements were performed with the devices granted by Gazi University Research Foundation, Project No. 01/2003-62 and 01/2003–62. Biochemical, histological, and genetic analyzes were performed at the Biotechnology Institute of Ankara University and the Faculty of Medicine of Gazi University.en_US
dc.identifier.doi10.1080/15368378.2022.2117187
dc.identifier.endpage401en_US
dc.identifier.issn15368378
dc.identifier.issue4en_US
dc.identifier.pmid36062506en_US
dc.identifier.scopus2-s2.0-85137841357en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage389en_US
dc.identifier.urihttps://doi.org/10.1080/15368378.2022.2117187
dc.identifier.urihttps://hdl.handle.net/20.500.14034/875
dc.identifier.volume41en_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherTaylor and Francis Ltd.en_US
dc.relation.journalElectromagnetic Biology and Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectadaptive responseen_US
dc.subjectbleomycinen_US
dc.subjectDNA oxidative damageen_US
dc.subjectRadiofrequency radiationen_US
dc.subjectstress responseen_US
dc.subjectAdaptive control systemsen_US
dc.subjectAntioxidantsen_US
dc.subjectDNAen_US
dc.subjectGene expressionen_US
dc.subjectGlobal system for mobile communicationsen_US
dc.subjectMammalsen_US
dc.subjectPeptidesen_US
dc.subjectSuperoxide dismutaseen_US
dc.subjectTissueen_US
dc.subjectAdaptive responseen_US
dc.subjectBleomycinsen_US
dc.subjectDNA oxidative damageen_US
dc.subjectDNA-repairen_US
dc.subjectGenes expressionen_US
dc.subjectOxidative damageen_US
dc.subjectRadio frequency fieldsen_US
dc.subjectRadio frequency radiationen_US
dc.subjectRepair mechanismen_US
dc.subjectStresses responseen_US
dc.subjectCell deathen_US
dc.subjectadenosine diphosphateen_US
dc.subjectantioxidanten_US
dc.subjectbleomycinen_US
dc.subjectcatalaseen_US
dc.subjectDNA glycosyltransferaseen_US
dc.subjectDNA nucleotidylexotransferaseen_US
dc.subjectguanineen_US
dc.subjectnicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase inhibitoren_US
dc.subjectprotein p53en_US
dc.subjectreactive oxygen metaboliteen_US
dc.subjectriboseen_US
dc.subjectsuperoxide dismutaseen_US
dc.subjectanimalen_US
dc.subjectapoptosisen_US
dc.subjectDNA damageen_US
dc.subjectDNA repairen_US
dc.subjectgeneticsen_US
dc.subjectmaleen_US
dc.subjectmetabolismen_US
dc.subjectmouseen_US
dc.subjectoxidative stressen_US
dc.subjectAdenosine Diphosphateen_US
dc.subjectAnimalsen_US
dc.subjectAntioxidantsen_US
dc.subjectApoptosisen_US
dc.subjectBleomycinen_US
dc.subjectCatalaseen_US
dc.subjectDNA Damageen_US
dc.subjectDNA Glycosylasesen_US
dc.subjectDNA Nucleotidylexotransferaseen_US
dc.subjectDNA Repairen_US
dc.subjectGuanineen_US
dc.subjectMaleen_US
dc.subjectMiceen_US
dc.subjectOxidative Stressen_US
dc.subjectPoly(ADP-ribose) Polymerase Inhibitorsen_US
dc.subjectReactive Oxygen Speciesen_US
dc.subjectRiboseen_US
dc.subjectSuperoxide Dismutaseen_US
dc.subjectTumor Suppressor Protein p53en_US
dc.titleInvestigation of oxidative damage, antioxidant balance, DNA repair genes, and apoptosis due to radiofrequency-induced adaptive response in miceen_US
dc.typeArticleen_US

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