Optimizing near infrared laser irradiation and photosensitizer accumulation period for indocyanine green-mediated photodynamic therapy in breast cancer xenografts: a focus on treatment and characterization

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dc.authoridTabakoglu, Hasim Ozgur/0000-0001-7239-9856
dc.contributor.authorTabakoglu, Hasim Ozgur
dc.contributor.authorAydogan, Tugba Kiris
dc.contributor.authorKiris, Aysenur
dc.contributor.authorAkbulut, Saadet
dc.date.accessioned2025-03-20T09:51:20Z
dc.date.available2025-03-20T09:51:20Z
dc.date.issued2024
dc.departmentİzmir Bakırçay Üniversitesi
dc.description.abstractPhotodynamic therapy (PDT) is a promising cancer treatment approach. Indocyanine green (ICG) is a water-soluble tricarbocyanine dye with a peak absorption wavelength of around 800 nm and possesses the capacity to produce reactive oxygen species. FTIR spectroscopy is rarely used and offers insights into molecular changes in cancer studies. MCF-7 cells were injected into Nude mouse. Once the tumor had grown to a size of 3-4 mm, mice were randomized into the 12 PDT groups. After each mouse received 5 mg/kg of ICG, they were photo-irradiated with a diode laser emitting light at 809 nm, followed by waiting intervals of 0, 30, 60, and 90 min. Laser irradiation parameters were 150, 250, 500 mW/cm2 and irradiation duration was 1200s. The tumor size was measured every day for four days. The FTIR spectroscopy was used to perform spectral analysis on tumor tissue samples. Four distinct regions (3600-2800 cm-1, 1750-1550 cm-1, 1540-1450 cm-1, and 1700-1100 cm-1) were analyzed, and Hierarchical Cluster study was carried out. A decrease in tumor volume was observed with all PDT applications, except, increases in tumor volume was observed at 150mW 90-minute group. PDT administered after 90 min revealed variations in 150mW and 250mW laser powers in the 3600 cm-1-2800 cm-1 range. The 250mW and 500mW applications resulted in a considerable reduction in fibroadenoma and carcinoma tissues, according to an analysis comparing the A1695 / A1635 ratio. It is proposed that the ideal treatments for further investigation have a power output of 250 mW.
dc.description.sponsorshipTUBITAK
dc.description.sponsorshipNo Statement Available
dc.identifier.doi10.1007/s10103-024-04202-z
dc.identifier.issn0268-8921
dc.identifier.issn1435-604X
dc.identifier.issue1
dc.identifier.pmid39382719
dc.identifier.scopus2-s2.0-85206236787
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s10103-024-04202-z
dc.identifier.urihttps://hdl.handle.net/20.500.14034/2491
dc.identifier.volume39
dc.identifier.wosWOS:001329339400001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer London Ltd
dc.relation.ispartofLasers in Medical Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250319
dc.subjectPhotodynamic therapy
dc.subjectIndocyanine green
dc.subjectFTIR spectroscopy
dc.subjectMCF-7
dc.titleOptimizing near infrared laser irradiation and photosensitizer accumulation period for indocyanine green-mediated photodynamic therapy in breast cancer xenografts: a focus on treatment and characterization
dc.typeArticle

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