A new technology for the treatment of premature ovarian insufficiency: Cell sheet

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dc.authoridYılmaz, Canan/0000-0002-6799-6522
dc.authoridCalis, Pinar/0000-0001-9334-1987
dc.authorwosidYılmaz, Canan/AAT-7788-2020
dc.contributor.authorCalis, Pinar
dc.contributor.authorArik, Gokcenur
dc.contributor.authorSeymen, Cemile Merve
dc.contributor.authorBayrak, Gokce Kaynak
dc.contributor.authorAkdere, Ozge Ekin
dc.contributor.authorYilmaz, Canan
dc.contributor.authorSaglam, Atiye Seda Yar
dc.date.accessioned2024-03-09T18:48:15Z
dc.date.available2024-03-09T18:48:15Z
dc.date.issued2024
dc.departmentİzmir Bakırçay Üniversitesien_US
dc.description.abstractPremature ovarian insufficiency (POI) is defined as the development of hypergonadotropic hypogonadism before the age of 40 with definitive treatment being absent. In the current study, we aim to compare the efficacy of the cell sheet method with an intravenous (IV) application of adipose-derived mesenchymal stem cells (AdMSCs) to the POI with an animal model. In the current prospective study, 6-to-8-week-old Sprague Dawley rats were generated four groups: (i) a control group in which only PBS was administered; (ii) an only-POI group generated by cyclophosphamide; (iii) a POI group treated by way of IV AdMSCs; and (iv) a POI group treated by way of the cell sheet method. Twenty-eight days after an oophorectomy was performed, intracardiac blood was taken. Follicle count, immunohistochemical examination for GDF9, BMP15, and TUNEL were conducted, gene expressions of GDF9 and BMP15 were examined, and E2 was measured in the serum samples. With hematoxylin-eosin, in the third group, multi oocytes follicles were the most remarkable finding. In the fourth group, most of the follicles presented normal morphology. GDF9 involvement was similar between the first and fourth groups. BMP-15 immunoreactivity, in contrast to fourth group, was weak in all stages in the second and third groups. The current attempt represents a pioneer study in the literature in which a cell sheet method is used for the first time in a POI model. These results suggest that the cell sheet method may be a feasible and efficient method for the stem cell treatment of models with POI and could be a new treatment approach in POI.en_US
dc.description.sponsorshipTSRM 2018en_US
dc.description.sponsorshipThe authors wish to thank the TSRM and TAJEV societies for their support.en_US
dc.identifier.doi10.1007/s00441-023-03848-5
dc.identifier.endpage131en_US
dc.identifier.issn0302-766X
dc.identifier.issn1432-0878
dc.identifier.issue1en_US
dc.identifier.pmid38049591en_US
dc.identifier.scopus2-s2.0-85178444997en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage117en_US
dc.identifier.urihttps://doi.org/10.1007/s00441-023-03848-5
dc.identifier.urihttps://hdl.handle.net/20.500.14034/1252
dc.identifier.volume395en_US
dc.identifier.wosWOS:001112823900001en_US
dc.identifier.wosqualityN/Aen_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofCell and Tissue Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPremature Ovarian Insufficiency; Stem Cell; Cell Sheet; Gdf9; Bmp15en_US
dc.titleA new technology for the treatment of premature ovarian insufficiency: Cell sheeten_US
dc.typeArticleen_US

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