COMPARISON OF SOME COPPER COMPOUNDS IN TERMS OF THEIR INTERACTIONS WITH DNA USING AGAROSE GEL ELECTROPHORESIS, UV-ABSORBANCE AND FLUORESCENCE SPECTROPHOTOMETRY TECHNIQUES

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dc.authorscopusid57862987100
dc.authorscopusid12770830300
dc.authorscopusid57217017730
dc.authorscopusid24921796800
dc.contributor.authorAkanbong E.A.
dc.contributor.authorDevrim A.K.
dc.contributor.authorŞenol A.
dc.contributor.authorSudagidan M.
dc.date.accessioned2024-03-09T19:40:02Z
dc.date.available2024-03-09T19:40:02Z
dc.date.issued2023
dc.departmentİzmir Bakırçay Üniversitesien_US
dc.description.abstractIn this study, to investigate the interaction of genomic DNA with some copper compounds (CuSO4, CuCO3, and CuCl2) at various concentrations (1000 ?M, 500 ?M, 250 ?M, 125 ?M and 62.5 ?M); UV-absorbance spectrophotometry, agarose gel electrophoresis, and fluorescence spectrophotometry techniques were used. When the UV-spectrophotometry data were examined within the wavelength range of 220-320 nm, the hyperchromic effect of CuCl2 was evaluated to be proportional to its concentrations. Within the wavelength range, the copper compounds produced their strongest hyperchromic effect on the DNA at 1000 ?M. When the fluorescent spectrophotometry data were evaluated, the hypochromic effect of CuCl2 within the wavelength range of 400-700 nm was determined at 1000 ?M. CuSO4 showed a hypochromic effect at concentrations above 62.5 ?M. Also, it was observed that CuCO3 did not interact with DNA. According to the agarose gel electrophoresis findings, the copper compounds investigated decreased DNA band intensity as their concentrations increased. It was observed that the most significant decrease in band intensity was caused by CuCl2 at 1000 ?M. Again, it was observed that the copper compounds did not cause any cleavage in the genomic DNA within the concentration range 62.5-1000 ?M. Consequently, the copper compounds bind to DNA, most probably by non-intercalative mode. In this regard, they could have the potential to be used in the development of new therapeutic agents. Thence, conducting further studies on the interaction of the copper compounds with DNA, their possible DNA uncoiling activities, and also the investigation of these compounds in cancer cell lines will provide useful results. © 2023 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.en_US
dc.identifier.endpage30en_US
dc.identifier.issn1511-2616
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85175253186en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage22en_US
dc.identifier.urihttps://hdl.handle.net/20.500.14034/1636
dc.identifier.volume26en_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMalaysian Society for Biochemistry and Molecular Biologyen_US
dc.relation.ispartofMalaysian Journal of Biochemistry and Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAgarose gel electrophoresis; Copper compounds; DNA-metal interaction; Fluorescence spectrophotometry; Genomic DNA; UV-absorbanceen_US
dc.titleCOMPARISON OF SOME COPPER COMPOUNDS IN TERMS OF THEIR INTERACTIONS WITH DNA USING AGAROSE GEL ELECTROPHORESIS, UV-ABSORBANCE AND FLUORESCENCE SPECTROPHOTOMETRY TECHNIQUESen_US
dc.typeArticleen_US

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