Yazar "Zeytinli Aksit, Merve" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ X ]
    Öğe
    Evaluation of measurement uncertainty of coagulation parameters according to two different current guidelines
    (Association of Clinical Biochemistry Specialists (Klinik Biyokimya Uzmanlari Dernegi), 2025) Zeytinli Aksit, Merve
    Objectives: This study aims to calculate the measurement uncertainty values of prothrombin time (PT), activated partial thromboplastin time (APTT), D-dimer, and fibrinogen tests according to ISO/TS 20914 and Nordtest 2017 guidelines and to compare these values with the total allowable error (TEa%) and maximum expanded allowable measurement uncertainty (MAU) values established by international organizations. Methods: Normal and pathological level internal quality control data for PT, APTT, D-dimer, and fibrinogen tests performed on the Sysmex CS2100 device between January and May 2024 were obtained from the Laboratory Information System. External quality control data for October 2023 and September 2024 were sourced from the external quality control system. Measurement uncertainty was calculated following ISO/TS 20914 and Nordtest 2017 guidelines. Results: According to the ISO/TS 20914 guideline, the measurement uncertainty values for PT, APTT, D-dimer, and fibrinogen tests were 10.42%, 3.49%, 4.81%, and 19.10%, respectively. According to the Nordtest guideline, the measurement uncertainty values were 10.44%, 12.64%, 17.94%, and 21.69%, respectively. Conclusion: Based on the ISO/TS 20914 guideline, it was observed that the measurement uncertainty values for all coagulation tests met the TEa% analytical targets. According to the Nordtest guideline, all tests except fibrinogen met these targets. When evaluated against the MAU criterion, it was determined that D-dimer met the targeted quality specification according to both guidelines; however, the measurement uncertainty values for PT, APTT, and fibrinogen exceeded the allowed targets. Standardization of the measurement uncertainty calculation model and the determination of analytical targets based on laboratory priorities can ensure reliable monitoring of analytical performance. © 2025, Association of Clinical Biochemistry Specialists (Klinik Biyokimya Uzmanlari Dernegi). All rights reserved.
  • Küçük Resim
    Öğe
    Stability of potassium, calcium and phosphorus electrolytes in three different tubes in patients with essential thrombocytosis
    (Taylor & Francis Ltd, 2023) Aksit, Murat; Zeytinli Aksit, Merve; Colak, Ayfer; Isbilen Basok, Banu; Ceylan, Cengiz
    Proper blood collection and timely analysis are vital steps for reliable results. This study aims to compare potassium(K), calcium(Ca), and phosphorus(P) concentrations in serum separator tube (SST), lithium heparin tube without gel (LiH), and lithium heparin tube with a barrier (Barricor)tubes in essential thrombocytosis(ET) patients. Additionally, we assessed short-term stability of these analytes at room temperature. K, Ca and P concentrations of blood taken from 40 ET patients into SST, LiH and Barricor tubes were measured at 0, 2, 4 and 8 h. We calculated the percentage difference and defined the maximum permissible difference (MPD) using the Biological Variation Database. Intertube comparisons were conducted using Passing-Bablok regression and Bland-Altman analysis. Comparing SST to LiH, the percentage difference values for all tests exceeded the MPD. When comparing Barricor to LiH, K and Ca tests were above MPD, except for P. At the 8th hour, LiH showed clinically significant changes in all three electrolytes. Barricor exhibited stability for K, Ca, and P for up to 8 h, with only Ca levels borderline higher than the MPD. Our study reveals clinically significant alterations in K, Ca, and P concentrations in SST compared to LiH tubes, and in K and Ca concentrations in Barricor compared to LiH tubes. While K, Ca and P concentrations were stable for up to 4 h at room temperature in all tube types tested, significant changes were observed in all electrolytes at 8 h in the LiH tube.