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    COMPARISON OF SOME COPPER COMPOUNDS IN TERMS OF THEIR INTERACTIONS WITH DNA USING AGAROSE GEL ELECTROPHORESIS, UV-ABSORBANCE AND FLUORESCENCE SPECTROPHOTOMETRY TECHNIQUES
    (Malaysian Society for Biochemistry and Molecular Biology, 2023) Akanbong E.A.; Devrim A.K.; Şenol A.; Sudagidan M.
    In this study, to investigate the interaction of genomic DNA with some copper compounds (CuSO4, CuCO3, and CuCl2) at various concentrations (1000 ?M, 500 ?M, 250 ?M, 125 ?M and 62.5 ?M); UV-absorbance spectrophotometry, agarose gel electrophoresis, and fluorescence spectrophotometry techniques were used. When the UV-spectrophotometry data were examined within the wavelength range of 220-320 nm, the hyperchromic effect of CuCl2 was evaluated to be proportional to its concentrations. Within the wavelength range, the copper compounds produced their strongest hyperchromic effect on the DNA at 1000 ?M. When the fluorescent spectrophotometry data were evaluated, the hypochromic effect of CuCl2 within the wavelength range of 400-700 nm was determined at 1000 ?M. CuSO4 showed a hypochromic effect at concentrations above 62.5 ?M. Also, it was observed that CuCO3 did not interact with DNA. According to the agarose gel electrophoresis findings, the copper compounds investigated decreased DNA band intensity as their concentrations increased. It was observed that the most significant decrease in band intensity was caused by CuCl2 at 1000 ?M. Again, it was observed that the copper compounds did not cause any cleavage in the genomic DNA within the concentration range 62.5-1000 ?M. Consequently, the copper compounds bind to DNA, most probably by non-intercalative mode. In this regard, they could have the potential to be used in the development of new therapeutic agents. Thence, conducting further studies on the interaction of the copper compounds with DNA, their possible DNA uncoiling activities, and also the investigation of these compounds in cancer cell lines will provide useful results. © 2023 Malaysian Society for Biochemistry and Molecular Biology. All rights reserved.