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    Assessing effects of caffeic acid on cytotoxicity, apoptosis, invasion, GST enzyme activity, oxidant, antioxidant status and micro-RNA expressions in HCT116 colorectal cancer cells
    (Elsevier, 2023) Secme, Mucahit; Mutlu, Dogukan; Elmas, Levent; Arslan, Sevki
    In developed western countries, colorectal cancer is one of the most prevalent cancer types. Natural bioactive compounds attract attention due to their antioxidant, anti-inflammatory, anticancer and therapeutic activities. Caffeic acid (3,4-dihydroxycinnamic) is one of the one of the most common phenolic acids found in many foods and plants such as coffee, honey, blueberries, herbs, potatoes, apples. The aim of the study is investigating cytotoxic, apoptotic, anti-invasive, oxidant, antioxidant, GST inhibitory activities of caffeic acid in colon cancer cells. Another aim of this study is to demonstrate a mechanistic approach by investigating the expression changes of mRNA and micro RNAs that play a role in these cellular mechanisms. In this context, MTT, Annexin-V, Matrigel invasion, TAS-TOS, GST enzyme activity assay and Real-Time PCR experiments were performed under in vitro conditions. According to results, caffeic acid have exhibits antiproliferative, apoptotic and anti-invasive activity in HCT116 cells. It caused changes expression of apoptosis, cell cycle and invasion-related genes and microRNAs. Caffeic acid also reduced oxidative stress and GST enzyme activity. Taken together, all these results reveal that caffeic acid may be a therapeutic agent in HCT116 colorectal cancer cells. This study will shed light on future comprehensive studies. (c) 2023 SAAB. Published by Elsevier B.V. All rights reserved.
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    Erianin, a promising agent in the treatment of glioblastoma multiforme triggers apoptosis in U373 and A172 glioblastoma cells
    (Inst Bioloska Istrazivanja Sinisa Stankovic, 2022) Kocoglu, Sema Serter; Secme, Mucahit; Elmas, Levent
    Glioblastoma is an aggressive, common and deadly primary intracranial brain tumor in adults. The antitumor activity of erianin, a dibenzyl compound found in Dendrobium chrysotoxum Lindl. extract, has not been previously demonstrated in glioblastoma. We investigated the anticancer activity and underlying mechanisms of erianin in human U373 and A172 glioma cells. The effects of erianin on cell viability, apoptosis, migration and invasion were estimated by the XTT test, the reverse transcription-polymerase chain reaction (RT-PCR), annexin V staining assay protocol for apoptosis, wound healing assay, and Matrigel (R) invasion chamber, respectively. The effective amounts of erianin in U373 and A172 cells were 16 and 64 mu M at 48 h, respectively. Erianin also significantly induced apoptosis by inhibiting B-cell lymphoma 2 (Bcl-2), caspase-8, caspase-9 and tumor necrosis factor receptor type 1-associated DEATH domain protein (TRADD), and activation of caspase-3 and BH3 interacting domain death agonist (BID) gene expression. In addition, erianin significantly increased the number of apoptotic cells in U373 and A172 cells and significantly decreased invasion and migration in U373 and A172 cells. Taken together, our results suggest that erianin may be a new therapeutic anticancer drug component with a potent apoptotic effect and a potential for treating glioblastoma.