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    Evaluation of Oxidative System Parameters in Alzheimer's Disease Before Medical Treatment
    (Taiwan Soc Geriatric Emergency & Critical Care Medicine-Tsgecm, 2022) Ayada, Ceylan; Erbay, Umran Toru; Korkut, Yasemin; Guleken, Zozan; Oner, Zulal
    Background: Alzheimer's disease (AD) is the most common reason for dementia and is one of the most important causes of morbidity and mortality in the aging population. A crucial component of AD is the brain's sensitivity to oxidative stress. We aimed to determine the oxidative load of patients with AD who had just been diagnosed and had not yet begun medical treatment.Methods: To assess oxidative load before drug administration, we compared the levels of serum total antioxidant (TAS), oxidant status (TOS), paraoxonase (PON1), arylesterase (ARES), total thiol (THIOL) levels in patients just diagnosed with AD (n = 41) and control (n = 45) with the totally 86 individuals. AD and control groups oxidative stress index (OSI) ratio was calculated too.Results: There was a statistically significant difference between the AD and control groups for mean TAS, TOS, and OSI levels with a 95% confidence level (pTAS = 0.001, pTOS = 0.005, pOSI = 0.001). There was not a statistically significant difference between the groups in terms of mean PON1, ARES, and THIOL values. Significantly negative and positive correlations were found for the interested parameters in both groups.Conclusion: The increase in antioxidative capacity in patients with AD may be related to ARES supported by TAS, and THIOL levels suggest, that those protein oxidation mechanisms are effective in the progress of AD disease before medication.Copyright (c) 2022, Taiwan Society of Geriatric Emergency & Critical Care Medicine.
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    FTIR, RAMAN and biochemical tools to detect reveal of oxidative Stress-Related lipid and protein changes in fibromyalgia
    (Elsevier, 2023) Guleken, Zozan; Suna, Gizem; Karaca, Sahika Burcu; Bulut, Huri; Ayada, Ceylan; Pancerz, Krzysztof; Paja, Wieslaw
    In this study, our aim was to investigate the pathogenesis and diagnosis of fibromyalgia (FM), a complex disorder with poorly understood causes. We focused on examining the role of oxidative stress and associated lipid and protein alterations in FM patients. To achieve this, we conducted a comprehensive analysis of serum samples obtained from 60 FM patients and 40 healthy individuals. In our analysis, we employed various biochemical assays and spectroscopic techniques including Fourier Transform Infra-Red (FTIR) and Raman spectroscopy. Moreover, we applied advanced statistical methods such as chemometrics and machine learning algorithms to analyze the collected data.The obtained results showed higher levels of oxidative stress, around 113% on the visual analogue scale score and around 5800% higher when C = O vibrations from lipids visible in FTIR spectra were analyzed. Also, lower levels of total antioxidants and oxidants in FM patients were observed compared with the healthy group.Moreover, FTIR spectra of serum collected from FM patients showed significantly higher absorbance of bands corresponding to polysaccharides, proteins, and lipids, while differences were not found in the Raman spectra. The principal component analysis (PCA) of the obtained spectroscopic data showed that it is possible to distinguish patients suffering from FM and healthy control groups with 100% accuracy using FTIR spectroscopy. PLS analysis showed significance in the differentiation of lipid vibrations among groups. In summary, FTIR coupled with chemometrics has the potential for fibromyalgia diagnosis.